Abstract:
This study focuses on attempting to reproduce the ideal conditions for
carpogenic germination of Sclerotinia sclerotiorum, a notable agricultural pathogen,
in a BOD chamber. The research was meticulous in creating a controlled
environment, however, the results did not match the expectations. The high humidity
in the BOD chamber prevented the formation of apothecia, fundamental structures
for the sexual reproduction of the fungus. This result underlines the delicacy and
complexity of the carpogenic germination process of S. sclerotiorum. It became evident that the precise balance between air and soil moisture is crucial for the
development of apothecia. In addition, the excess humidity may have negatively
impacted the sclerotia, contributing to the lack of success in germination. This study
highlights the intrinsic sensitivity of the process and the significant implications for
future investigations. Understanding these challenges is vital for advances in
pathogen biology and for enhancing management strategies in real agricultural
environments. Therefore, this study highlights the need for precise considerations of
environmental conditions when designing experiments aimed at understanding the
carpogenic germination of S. sclerotiorum.
